Coding

Part:BBa_K2314106:Design

Designed by: liu chenguang   Group: iGEM17_OUC-China   (2017-10-22)


This part is a gene which encoding a cellobiose transporter(CDT-1).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 484
    Illegal NotI site found at 602
    Illegal NotI site found at 962
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
    Illegal XhoI site found at 1432
    Illegal XhoI site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1024
    Illegal BsaI.rc site found at 1435
    Illegal BsaI.rc site found at 1579
    Illegal SapI site found at 945


Design Notes

NO


Source

CDT-1(cellodextrin transporter) from the filamentous fungus N.crassa Ref: Nobuhiro Nakamura, Ryosuke Yamada, Satoshi Katahira, Tsutomu Tanaka, Hideki Fukuda, & Akihiko Kondo. (2008). Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating s. cerevisiae, via expression of β-glucosidase on its cell surface. Enzyme & Microbial Technology, 43(3), 233-236. Galazka, J. M., et al. "Cellodextrin transport in yeast for improved biofuel production." Science 330.6000(2010):84.

References